Results
| PMID | 26530052 |
| Gene Name | NR5A1 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 44 |
| Population details | 44 (16 patients with endometriosis and 28 controls) |
| Sex | Female |
| Infertility type | Female infertility |
| Associated genes | CYP19, PII, LRH-1 and SF-1 |
| Other associated phenotypes |
Endometriosis |
|
J Obstet Gynaecol Res. 2015 Dec;41(12):1927-34. doi: 10.1111/jog.12832. Epub 2015 Lu, Xiang| Wu, Zheng-mu| Wang, Yong-wei| Wang, Min| Cheng, Wei-wei Reproductive Medical Center, International Peace Maternity and Child Health Hospital Affiliated, Shanghai Jiao Tong University, School of Medicine, Shanghai, China.| Reproductive Medical Center, International Peace Maternity and Child Health Hospital AIM: The expression of aromatase (via CYP19 and the CYP19 PII promoter) and the orphan nuclear receptor family members, liver receptor homologue-1 (LRH-1) and steroidogenic factor-1 (SF-1) in cultured luteinized granulosa cells from women with endometriosis were investigated. METHODS: Luteinized granulosa cells from patients undergoing in vitro fertilization (16 patients with endometriosis and 28 controls) were examined for messenger ribonucleic acid (mRNA) expression of CYP19, CYP19 PII, LRH-1 and SF-1, determined by quantitative reverse transcription-polymerase chain reaction. Student's t-test, the Mann-Whitney U test and Spearman's rank correlation were used for statistical analysis. RESULTS: The number of high quality embryos in the endometriosis group was significantly lower than in the control group. The mRNA expression levels of CYP19, CYP19 PII, LRH-1 and SF-1 in granulosa-lutein cells were decreased in women with endometriosis compared to the control group. The simultaneous down-regulation expression of LRH-1, SF-1 and CYP19 PII in endometriotic granulosa cells indicated their positive correlation. CONCLUSION: Our results demonstrate aberrant expressions of SF-1 and LRH-1 in endometriotic granulosa-lutein cells. This finding may be helpful in understanding infertility associated with endometriosis and reduced P450 aromatase activity in endometriotic granulosa cells. Mesh Terms: Adult| Aromatase/genetics| Cells, Cultured| Endometriosis/*metabolism| Female| Granulosa Cells/*metabolism| Humans| RNA, Messenger/analysis| Receptors, Cytoplasmic and Nuclear/*genetics| Steroidogenic Factor 1/*genetics|DA 2017/01/12 06:00 |
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